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  • Title: [Analysis of 16S rDNA sequence of Cronobacter spp].
    Author: Pei X, Liu X.
    Journal: Wei Sheng Yan Jiu; 2010 Jan; 39(1):36-9. PubMed ID: 20364584.
    Abstract:
    OBJECTIVE: To analyse the sequence of 16S rDNA of twenty-nine Cronobacter spp. strains isolated from food as well as two type strains. METHODS: Using polymerase chain reaction to obtain the sequences of 16S rDNA of all strains. The multiple cluster analysis and phylogenetic analysis of them were studied by BioNumerics software. RESULTS: All strains, except ATCC51329 and ES 014, were clustered with the Cronobacter spp. type strain ATCC29544. The Cronobacter spp. type strain ATCC51329 and ES 014 belonged to a different branch. From the sequence analysis of 16S rDNA, all these isolates of Cronobacter spp. except ES 014 could be identified accurately by gene analysis. CONCLUSION: All Cronobacter spp. strains isolated from food were identified on genetic level. The phylogenetic relationships among related Cronobacter spp. isolates were studied.
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