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  • Title: High-resolution x-ray diffraction microscopy of specifically labeled yeast cells.
    Author: Nelson J, Huang X, Steinbrener J, Shapiro D, Kirz J, Marchesini S, Neiman AM, Turner JJ, Jacobsen C.
    Journal: Proc Natl Acad Sci U S A; 2010 Apr 20; 107(16):7235-9. PubMed ID: 20368463.
    Abstract:
    X-ray diffraction microscopy complements other x-ray microscopy methods by being free of lens-imposed radiation dose and resolution limits, and it allows for high-resolution imaging of biological specimens too thick to be viewed by electron microscopy. We report here the highest resolution (11-13 nm) x-ray diffraction micrograph of biological specimens, and a demonstration of molecular-specific gold labeling at different depths within cells via through-focus propagation of the reconstructed wavefield. The lectin concanavalin A conjugated to colloidal gold particles was used to label the alpha-mannan sugar in the cell wall of the yeast Saccharomyces cerevisiae. Cells were plunge-frozen in liquid ethane and freeze-dried, after which they were imaged whole using x-ray diffraction microscopy at 750 eV photon energy.
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