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  • Title: Inbred Chinese Wuzhishan (WZS) minipig model for soybean glycinin and beta-conglycinin allergy.
    Author: Huang Q, Xu H, Yu Z, Gao P, Liu S.
    Journal: J Agric Food Chem; 2010 Apr 28; 58(8):5194-8. PubMed ID: 20369852.
    Abstract:
    Validated murine models have been built to assess the potential allergenicity of novel proteins. Large animals, such as pigs, share more similarities to humans in physiology and immunology than murine. Among Chinese minipigs, Wuzhishan (WZS) minipigs have the highest inbreeding coefficient, more stable heredity, and less variability, which were gastrically sensitized and excitated with diets containing 4% glycinin or 4% beta-conglycinin or neither to induce anaphylactic reactions in the present study. In glycinin- and beta-conglycinin-sensitized animals, diarrhea symptoms and skin wheal and flare responses were observed. In comparison to the control, after oral excitation with glycinin or beta-conglycinin, the serum IgE was increased by 34.4 or 38.4% and the serum histamine was increased by 42.1 or 46.9%, respectively. In addition, the serum IFN-gamma were reduced by 12.4 or 30.0%, respectively. The jejunum histamine level of beta-conglycinin-sensitized animals was increased by 196.6%, while the number of mast cells in the submucosa of jejunum and ileum of the glycinin-sensitized animals declined by 48.1 and 45.0%. In conclusion, the WZS minipig allergy models induced by soybean glycinin and beta-conglycinin represent type-I hypersensitivity reactions mediated by IgE, which could potentially be useful in determining the potential allergenicity of novel proteins.
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