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  • Title: Purification and partial characterization of glyoxalase I from a higher plant Brassica juncea.
    Author: Deswal R, Sopory SK.
    Journal: FEBS Lett; 1991 May 06; 282(2):277-80. PubMed ID: 2037046.
    Abstract:
    The glyoxalase I was purified from Brassica juncea by affinity chromatography on S-hexyl GSH sepharose 4B. Homogeneity of the protein was confirmed electrophoretically by a silver stained gel. Activity staining on a native starch gel also showed a single band. The effect of glutathione, methylglyoxal, and pH on enzyme kinetics was studied. Magnesium was found to stimulate the enzyme activity.
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