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  • Title: Kinetics of D-lactic acid production by Sporolactobacillus sp. strain CASD using repeated batch fermentation.
    Author: Zhao B, Wang L, Li F, Hua D, Ma C, Ma Y, Xu P.
    Journal: Bioresour Technol; 2010 Aug; 101(16):6499-505. PubMed ID: 20374976.
    Abstract:
    D-lactic acid was produced by Sporolactobacillus sp. strain CASD in repeated batch fermentation with one- and two-reactor systems. The strain showed relatively high energy consumption in its growth-related metabolism in comparison with other lactic acid producers. When the fermentation was repeated with 10% (v/v) of previous culture to start a new batch, D-lactic acid production shifted from being cell-maintenance-dependent to cell-growth-dependent. In comparison with the one-reactor system, D-lactic acid production increased approximately 9% in the fourth batch of the two-reactor system. Strain CASD is an efficient D-lactic acid producer with increased growth rate at the early stage of repeated cycles, which explains the strain's physiological adaptation to repeated batch culture and improved performance in the two-reactor fermentation system. From a kinetic point of view, two-reactor fermentation system was shown to be an alternative for conventional one-reactor repeated batch operation.
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