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  • Title: Rational creation of mutant enzyme showing remarkable enhancement of catalytic activity and enantioselectivity toward poor substrates.
    Author: Ema T, Kamata S, Takeda M, Nakano Y, Sakai T.
    Journal: Chem Commun (Camb); 2010 Aug 14; 46(30):5440-2. PubMed ID: 20383389.
    Abstract:
    Catalytic activity and enantioselectivity of lipase toward poor substrates bearing bulky substituents on both sides have been dramatically improved by rational design; the E value for a poor substrate was increased from 5 (wild-type enzyme) to >200 (I287F/I290A double mutant) with an acceleration of the reaction rate.
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