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  • Title: [Development a method of quantitative assay for enterovirus 71].
    Author: Xu J, Cui WY, Li SX, Liu J, Wang XY, Chen L, Shen XL.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2009 Oct; 23(5):388-90. PubMed ID: 20387495.
    Abstract:
    OBJECTIVE: To establish a quantitative assay for enterovirus 71, this can be used in detecting the virus content during vaccine development and production. METHODS: We established the method of quantitative assay for EV71 by using double antibody sandwich ELISA. The sensitivity, accuracy,precision and specificity of the method were evaluated. RESULTS: We developed an ELISA method to quantitative assay for EV71. The quantitation limit of the method is 0.23 microg/ml and the quantitation scope of the method is 7.32-0.23 microg/ml, the coefficient correlation is R2 = 0.9976; The method showed good accuracy, precision and specificity. The recovery is between 90%-110% and the variation coefficient is lower than 10%. CONCLUSION: An ELISA method was developed for the quantitative assay of EV71 virus, which can be used for the rapid quantitative determination of EV71 virus during vaccine development and production.
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