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  • Title: [Biosynthesis of the bioprotectant ectoin by aerobic methylotrophic bacteria from methanol].
    Author: Doronina NV, Ezhov VA, Beschastnyĭ AP, Trotsenko IuA.
    Journal: Prikl Biokhim Mikrobiol; 2010; 46(2):187-90. PubMed ID: 20391762.
    Abstract:
    It is shown that neutrophilic methylobacteria Methylophaga thalassica and M. marina have higher rates of growth and ectoin accumulation compared to the haloalkaliphilic species M. alcalica and M. natronia and methanotrophs Methylomicrobium alcaliphilum and M. kenyense. The conditions of M. thalassica cultivation in methanol-containing medium were optimized. The yield of this process reached 60 g/l of absolutely dry biomass containing 15-19% (9-11 g/l) ectoine. The scheme of ectoin isolation from the biomass by extraction and subsequent purification, which allowed obtaining preparations of different degree of purity, was developed.
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