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  • Title: Improvement of human interferon alpha secretion by Lactococcus lactis.
    Author: Zhang Q, Zhong J, Liang X, Liu W, Huan L.
    Journal: Biotechnol Lett; 2010 Sep; 32(9):1271-7. PubMed ID: 20431909.
    Abstract:
    To improve the secretion and expression of human interferon alpha 2b (IFN) in Lactococcus lactis, a synthetic pro-peptide, LEISSTCDA (LEISS), was fused to the N-terminus of IFN. This gave a higher secretion efficiency (12% vs. 5%) and yield (approximately 2.8-fold) of IFN. The signal peptide, SP(SlpA) (SlpA, an S-layer protein of Lactobacillus brevis), was also tested to secrete IFN instead of SP(Usp45) (Usp45, the main secreted protein in L. lactis). This gave increased IFN secretion (approximately 3-fold) but lower total production. All the recombinant IFN had appropriate bioactivities in an antiviral assay.
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