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  • Title: Solvent modulation in hydrophobic interaction chromatography.
    Author: Arakawa T, Narhi LO.
    Journal: Biotechnol Appl Biochem; 1991 Apr; 13(2):151-72. PubMed ID: 2043277.
    Abstract:
    Solvents play a critical role in hydrophobic interaction chromatography (HIC), since the separation of proteins by HIC is based on the hydrophobicity of the proteins presented to the solvents. This review first describes the solvent properties which determine the effect of cosolvents on the binding and elution of proteins in HIC; i.e., the protein solvent interactions and the surface tension of water/cosolvent mixture. Second are presented the various cosolvents which have been tested as facilitating binding or elution of the proteins. Last, some examples of solvent manipulation which resolved complex mixtures of proteins by HIC are reviewed.
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