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  • Title: Transfer and expression of a Streptomyces cholesterol oxidase gene in Streptococcus thermophilus.
    Author: Somkuti GA, Solaiman DK, Johnson TL, Steinberg DH.
    Journal: Biotechnol Appl Biochem; 1991 Apr; 13(2):238-45. PubMed ID: 2043280.
    Abstract:
    The recombinant plasmid pNCO937 (8.1 kbp) containing a Streptomyces sp. cholesterol oxidase gene was introduced into Streptococcus thermophilus by electrotransformation. Transformation frequency was 7.2 x 10(5) colony forming units/micrograms of DNA. The presence of the cholesterol oxidase gene in S. thermophilus was confirmed with Southern blot analysis using a biotinylated probe. Thin-layer chromatographic analysis showed the expression of the Streptomyces cholesterol oxidase gene resulting in the oxidation of cholesterol to 4-cholesten-3-one. S. thermophilus may be a suitable host for the expression of other genes regulating prokaryotic cholesterol metabolism.
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