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  • Title: Molecularly imprinted polymer-based chemiluminescence array sensor for the detection of proline.
    Author: Chang P, Zhang Z, Yang C.
    Journal: Anal Chim Acta; 2010 May 07; 666(1-2):70-5. PubMed ID: 20433967.
    Abstract:
    A new molecularly imprinted polymer (MIP)-chemiluminescence (CL) method has been developed for detection of proline. The molecularly imprinted polymer microspheres were synthesized using precipitation polymerization with hydroxyproline, a structural analogue, as the template. Polymer microspheres were immobilized in microtiter plates (96 wells) which selectively adsorbed the analyte (dansyl-proline). After washing, the bound fraction was quantified based on peroxyoxalate chemiluminescence (PO-CL) reaction enhanced by imidazole. The cavity of MIP synthesized with hydroxyproline as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-proline. Under the optimum conditions, the relative CL intensity has a linear relationship with the concentration of proline in the range of 1x10(-6) mol L(-1) to 4x10(-5) mol L(-1), with a limit of detection 3x10(-7) mol L(-1) (3sigma). The relative standard deviation (R.S.D.) for proline (1x10(-6) mol L(-1), n=7) was 3.7%. The MIP-CL method can become a useful analytical technology for determination of proline in real sample.
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