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  • Title: Antibiotic immunosensing: determination of sulfathiazole in water and honey.
    Author: Jornet D, González-Martínez MA, Puchades R, Maquieira A.
    Journal: Talanta; 2010 Jun 15; 81(4-5):1585-92. PubMed ID: 20441943.
    Abstract:
    The development of two sensitive and selective immunosensors for sulfathiazole, using immunoreagents - haptens, polyclonal antibodies, enzyme conjugates - previously obtained and characterized, is presented. One of them is based on the competitive immunocomplex capture format making use of an immobilized protein A/G sorbent, while the other employs a restricted access support in a novel homogeneous-heterogeneous (HH) assay mode. Maximum sensitivity, achieved with a total assay time of 18min for the capture sensor, is traduced in a dynamic range from 0.4 to 24microgL(-1), with a lower limit of detection of 0.11microgL(-1), increasing to 1.2microgL(-1) when employing an accelerated capture assay protocol that yields a sampling rate of 7 cycles per hour. The HH sensor shows the fastest response, performing each whole assay in only 2min, with a limit of detection of 0.85 and a measurement interval of 3.9-181.0microgL(-1), and with no need of support regeneration. Immunosensors are selective for sufathiazole, and only sulfamethoxypyridazine, sulfamethizole and sulfapyridine show non-negligible cross-reactivity, the same as in ELISA batch immunoassay. The application of the developed systems to the analysis of water, with no sample treatment, as well as honey samples after solid-phase extraction, demonstrate the reliability of the immunosensing for the monitoring of this type of pollutants.
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