These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Chiral discrimination between D- and L-tryptophan based on the alteration of the fluorescence lifetimes by the chiral additives.
    Author: Wei Y, Wang S, Shuang S, Dong C.
    Journal: Talanta; 2010 Jun 15; 81(4-5):1800-5. PubMed ID: 20441976.
    Abstract:
    The chiral microenvironmental properties of bovine serum albumin, cyclodextrins and their mixtures were comparatively investigated based on pyrene as the fluorescence probe. On this basis, a time resolved fluorescence technique to discriminate D- and L-tryptophan (Trp) without separation was developed. This method was based on the quenching rate difference between d- and l-Trp to pyrene in various media including bovine serum albumin, cyclodextrins and their mixtures. Pyrene, tryptophan and bovine serum albumin (or cyclodextrin) could form ternary complexes, which provided a possibility for the discrimination of D- and L-Trp enantiomers. The results indicated that the chiral discrimination of Trp enantiomers could be obtained in the mixture system of gamma-cyclodextrin and BSA system, in which the fluorescence lifetime difference (Deltatau) and the lifetime difference percentage (Deltatau/tau) were 55.8ns and 28.50%, respectively.
    [Abstract] [Full Text] [Related] [New Search]