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  • Title: Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation.
    Author: Lin H, Cheng W, Ding HT, Chen XJ, Zhou QF, Zhao YH.
    Journal: Bioresour Technol; 2010 Oct; 101(19):7556-62. PubMed ID: 20444596.
    Abstract:
    Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation (SSF) was investigated. In submerged fermentation, A. oryzae A-4 accumulated lipid to 15-18.15% of biomass when pure cellulose was utilized as the sole substrate. In SSF of the wheat straw and bran mixture, A. oryzae A-4 yielded lipid of 36.6mg/g dry substrate (gds), and a cellulase activity of 1.82 FPU/gds with 25.25% of holocellulose utilization in the substrates were detected on the 6th day. The lipid yield reached 62.87 mg/gds in SSF on the 6th day under the optimized conditions from Plackett-Burman design (PBD). Cellulase secretion of A. oryzae A-4 was found to influence the lipid yield. Dilute acid pretreatment of the straw and addition of some agro-industrial wastes to the straw could enhance lipid production of A. oryzae A-4.
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