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  • Title: Class II major histocompatibility complex restriction of human T cell responses to short ragweed allergen, Amb a V.
    Author: Huang SK, Zwollo P, Marsh DG.
    Journal: Eur J Immunol; 1991 Jun; 21(6):1469-73. PubMed ID: 2044656.
    Abstract:
    Although T cells are known to play a crucial role in the induction of IgE synthesis, the class II major histocompatibility complex (MHC) restriction of aeroallergen-induced T cell responses in humans is incompletely defined. We have previously shown that, in allergic Caucasoid individuals, HLA-DR2 and Dw2 (DR2.2) is strongly associated with specific IgE and IgG antibody responses to highly purified Ambrosia (ragweed) allergen, Amb a V, from the artemisiifolia (short) species. For example, 95% of IgE antibody responders to Amb a V were typed as DR2.2. In a novel study of the genetic control of T cell responses to the Amb a V allergen, we have investigated the MHC class II restriction specificity of three CD4, Amb a V-specific T cell clones derived from a DR2.2+ atopic patient, and a polyclonal Amb a V-reactive T cell line from another DR2.2+ patient. We observed proliferative responses of all three clones to Amb a V only when either HLA-DR2.2 or DR2, Dw12 (DR2.12; found on Mongoloid populations) was present on the antigen-presenting cells, regardless of the HLA-DQ phenotype of the cells. Moreover, the responses of T cell line and clones were abolished by anti-DR but not by anti-DQ nor by anti-DP monoclonal antibodies, and, significantly, anti-DR alpha/beta I2 (anti-DR alpha /beta Iw15/w16; anti-"DR2b") monoclonal antibody blocked, in a dose-dependent manner, the cloned T cell responses to Amb a V. These findings demonstrate that DR alpha/beta I2.2 (DR alpha/beta I1501) and DR alpha/beta I2.12 (DR alpha/beta I1502) are functional in the restriction of the T cell recognition of Amb a V. These findings also illustrate the power of the allergy model for definitive investigation of the molecular basis of the human immune response.
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