These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Induction of angiogenic immediate early genes by activation of FP prostanoid receptors in cultured human ciliary smooth muscle cells.
    Author: Hutchinson AJ, Coons SC, Chou CL, Xu W, Stamer WD, Woodward DF, Regan JW.
    Journal: Curr Eye Res; 2010 May; 35(5):408-18. PubMed ID: 20450254.
    Abstract:
    PURPOSE: Agonists of the F prostanoid receptor for prostaglandin F2alpha exert. exert an ocular hypotensive effect that has been attributed to increased aqueous humor outflow through the uveoscleral pathway. Although tissue remodeling of the ciliary muscle has been described, the signaling mechanisms that link activation of the FP receptor to remodeling of the ciliary muscle are poorly understood. Herein, we describe the identification of novel signaling mechanisms that may contribute to this process. MATERIALS AND METHODS: Cultures of human ciliary smooth muscle cells were established from fetal eye tissue explants. The cells were validated by their expression of alpha-smooth muscle-actin and functional FP receptors. Cultures were treated with prostaglandin F(2 alpha) and examined for the induction of three immediate early genes related to tissue remodeling using Western blot analysis, quantitative real-time polymerase chain reaction, and reporter gene assays. RESULTS: Human ciliary smooth muscle cells express functional FP receptors whose activation up-regulates the expression of early growth response factor-1 and connective tissue growth factor at the mRNA and protein levels. Prostaglandin F(2 alpha) stimulation also increases the protein expression of hypoxia-inducible factor-1 alpha and activates luciferase reporter plasmids under the control of the hypoxia response element. CONCLUSIONS: Early growth response factor-1 and hypoxia-inducible factor-1 alpha are important transcriptional activators of downstream genes involved in tissue remodeling and angiogenesis, whereas connective tissue growth factor is a secreted growth factor that also contributes to these processes. Thus, stimulation of FP receptors in human ciliary smooth muscle cells up-regulates the expression of immediate early genes that may coordinate the remodeling of the ciliary muscle, thereby facilitating aqueous outflow.
    [Abstract] [Full Text] [Related] [New Search]