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  • Title: Simultaneous biosynthesis of two copolymers in Pseudomonas putida GPo1 using a two-stage continuous culture system.
    Author: Hartmann R, Hany R, Witholt B, Zinn M.
    Journal: Biomacromolecules; 2010 Jun 14; 11(6):1488-93. PubMed ID: 20459087.
    Abstract:
    The bacterial production of medium-chain-length polyhydroxyalkanoate (PHA) in continuous two-stage cultivation was investigated. Two chemostats were linked in sequence and Pseudomonas putida GPo1 was supplied with different concentrations of octanoic acid in the first and 10-undecenoic acid in the second reactor. PHA isolated from the second bioreactor was a blend of two copolymers typically consisting of poly(3-hydroxyoctanoate-co-3-hydroxyhexanoate) (PHO) and poly(3-hydroxy-10-undecenoate-co-3-hydroxy-8-nonenoate-co-3-hydroxy-6-heptenoate) (PHUE) with structural (monomeric) purities of 85-95 mol %, indicating simultaneous PHA accumulation and degradation inside the cell. The maximum PHA content was found to be 53.8% of total cell dry weight, which is very high for mcl-PHA.
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