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  • Title: Ultrasensitive optical detection of hydrogen peroxide by triggered activation of horseradish peroxidase.
    Author: Virel A, Saa L, Köster SD, Pavlov V.
    Journal: Analyst; 2010 Sep; 135(9):2291-5. PubMed ID: 20464020.
    Abstract:
    Hydrogen peroxide is a very reactive byproduct of many metabolic pathways. We describe an ultra-sensitive colorimetric method to detect hydrogen peroxide based on the reconstitution of apo-horseradish peroxidase with the hemin derivative, hemin di(N,N'-acetyl-hydrazide). Oxidation of the latter by hydrogen peroxide yields hemin, which is able to reconstitute apo-horseradish peroxidase, forming an active peroxidase. We have also applied this analyte-triggered reconstitution of horseradish peroxidase to detect the activity of the enzyme glucose oxidase.
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