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  • Title: [Molecular genetic analysis of Pseudomonas aeruginosa strains isolated from environment and patients in health care facilities].
    Author: Antonov VA, Altukhova VV, Savchenko SS, Tkachenko GA, Zamaraev VS, Zhukova SI, Kramar' OG, Matveeva NL, Ostrovskiĭ OV, Dudchenko GP.
    Journal: Zh Mikrobiol Epidemiol Immunobiol; 2010; (2):8-13. PubMed ID: 20464993.
    Abstract:
    AIM: Analysis of genetic heterogeneity of Pseudomonas aeruginosa strains isolated in and out of hospitals. MATERIALS AND METHODS: To study the genetic diversity of 36 strains of P. aeruginosa plasmid analysis, random amplified polymorphic DNA (RAPD) technique as well as polymerase chain reaction for detection of virulence genes algD, lasB, toxA, plcH, plcN, exoS, nan1, and nan2. RESULTS: Epidemically important strains were found in different ecological niches. It was shown that these virulence factors could play important roles in pathogenesis of infection. CONCLUSION: RAPD technique was effective for analysis of P. aeruginosa isolates. Number of studied typing bands differed between related isolates for each random primer.
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