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  • Title: Fine structure of secondary granule inclusions in fowl heterophils after ruthenium tetroxide fixation.
    Author: Robertson GW, Maxwell MH.
    Journal: Res Vet Sci; 1991 Jan; 50(1):121-2. PubMed ID: 2047586.
    Abstract:
    Bone marrow from domestic fowls was initially fixed for electron microscopy in glutaraldehyde and post-fixed with either ruthenium tetroxide or osmium tetroxide. Inclusions with distinct outlines were revealed in the secondary granules of heterophil leucocytes after ruthenium tetroxide but not with osmium tetroxide fixation. In longitudinal orientation, these inclusions were rod-shaped and composed of microfilaments measuring 3.7 nm in diameter. In transverse section, the outline of some of these inclusions was hexagonal and therefore the inclusions may be crystalline in nature.
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