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  • Title: Mannose production from fructose by free and immobilized D-lyxose isomerases from Providencia stuartii.
    Author: Park CS, Kwon HJ, Yeom SJ, Oh DK.
    Journal: Biotechnol Lett; 2010 Sep; 32(9):1305-9. PubMed ID: 20480207.
    Abstract:
    A recombinant D-lyxose isomerase from Providencia stuartii was immobilized on Duolite A568 beads which gave the highest conversion of D-fructose to D-mannose among the various immobilization beads evaluated. Maximum activities of both the free and immobilized enzymes for fructose isomerization were at pH 7.5 and 45 degrees C in the presence of 1 mM Mn(2+). Enzyme half-lives were 14 and 30 h at 35 degrees C and 3.4 and 5.1 h at 45 degrees C, respectively. The immobilized enzyme in 300 g fructose/l (replaced hourly), produced 75 g mannose/l at 35 degrees C = 25% (w/w) yield with a productivity of 75 g mannose l(-1) h(-1) after 23 cycles.
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