These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: L-Ribulose production by an Escherichia coli harboring L-arabinose isomerase from Bacillus licheniformis.
    Author: Zhang YW, Jeya M, Lee JK.
    Journal: Appl Microbiol Biotechnol; 2010 Aug; 87(6):1993-9. PubMed ID: 20495916.
    Abstract:
    Recombinant Escherichia coli harboring the L: -arabinose isomerase (BLAI) from Bacillus licheniformis was used as a biocatalyst to produce L: -ribulose in the presence of borate. Effects of substrate concentration, the borate to L: -arabinose ratio, pH, and temperature on the conversion of L: -arabinose to L: -ribulose were investigated. L: -Ribulose production was efficient when pH was higher than 9 and temperature was higher than 50 degrees C. Borate addition to the reaction mixture was essential for high conversion of L: -arabinose to L: -ribulose as it resulted in an equilibrium shift in favor of the product. Under the optimal conditions determined by response surface methodology, the E. coli harboring BLAI produced 375 g l(-1) L-ribulose from 500 g l(-1) L: -arabinose at a reaction time of 60 min, corresponding to a conversion yield of 75% and productivity of 375 g l(-1) h(-1). When the resting recombinant E. coli cells were recycled, 85% of the yield was obtained even after seven cycles of reuse. The productivity and final concentration of L: -ribulose obtained in the present study were the highest yet reported.
    [Abstract] [Full Text] [Related] [New Search]