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  • Title: Application of a luminescent bacterial biosensor for the detection of tetracyclines in routine analysis of poultry muscle samples.
    Author: Pikkemaat MG, Rapallini ML, Karp MT, Elferink JW.
    Journal: Food Addit Contam Part A Chem Anal Control Expo Risk Assess; 2010 Aug; 27(8):1112-7. PubMed ID: 20496184.
    Abstract:
    Tetracyclines are extensively used in veterinary medicine. For the detection of tetracycline residues in animal products, a broad array of methods is available. Luminescent bacterial biosensors represent an attractive inexpensive, simple and fast method for screening large numbers of samples. A previously developed cell-biosensor method was subjected to an evaluation study using over 300 routine poultry samples and the results were compared with a microbial inhibition test. The cell-biosensor assay yielded many more suspect samples, 10.2% versus 2% with the inhibition test, which all could be confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Only one sample contained a concentration above the maximum residue limit (MRL) of 100 microg kg(-1), while residue levels in most of the suspect samples were very low (<10 microg kg(-1)). The method appeared to be specific and robust. Using an experimental set-up comprising the analysis of a series of three sample dilutions allowed an appropriate cut-off for confirmatory analysis, limiting the number of samples and requiring further analysis to a minimum.
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