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  • Title: Purification of Food Color Red No. 106 (acid red) using high-speed counter-current chromatography.
    Author: Oka H, Ikai Y, Kawamura N, Hayakawa J, Yamada M, Harada K, Murata H, Suzuki M, Nakazawa H, Suzuki S.
    Journal: J Chromatogr; 1991 Jan 18; 538(1):149-56. PubMed ID: 2050788.
    Abstract:
    High-speed counter-current chromatography (HSCCC) has been successfully applied to the separation of the components of Food Color Red No. 106 (R-106). The separation was performed using 25 mg of the sample with a two-phase solvent system composed of n-butanol and 0.01 M trifluoroacetic acid (1:1, v/v). Analyses by thin-layer chromatography, high-performance liquid chromatography and fast atom bombardment mass spectrometry confirmed that HSCCC was effective in the purification of the components of R-106. The separation gave 21 mg of a 99.9% pure main component (Acid Red) and 0.9 mg of 98.0% pure subsidiary dye which is probably a des-ethyl derivative.
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