These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Pectenotoxin-2 induces G2/M phase cell cycle arrest in human breast cancer cells via ATM and Chk1/2-mediated phosphorylation of cdc25C.
    Author: Moon DO, Kim MO, Nam TJ, Kim SK, Choi YH, Kim GY.
    Journal: Oncol Rep; 2010 Jul; 24(1):271-6. PubMed ID: 20514472.
    Abstract:
    Although pectenotoxin-2 (PTX-2) is known to regulate the actin depolymerization and to induce apoptosis through downregulation of telomerase activity, little is known on its effect on the cell cycle regulation. Therefore, we investigated the effects of PTX-2 on G2/M arrest in human breast cancer cells (MDA-MB-231 and MCF-7). Treatment with PTX-2 significantly suppressed cell proliferation and induced G2/M phase arrest through down-regulation of cyclin B1 and cdc2 expression, but also through phosphorylation of cdc25C. We found increased phosphorylation of ATM and Chk1/2 in a PTX-2 dose-dependent manner. Furthermore, treatment with PTX-2 increased H2O2 generation with correlated G2/M arrest. Our results showed that ATM- and Chk1/2-mediated phosphorylation of cdc25C plays a major role in G2/M arrest, but not in H2O2 generation induced by PTX-2 treatment. We also observed that PTX-2-induced cell cycle arrest was not restricted to p53 status in human breast cancer cells.
    [Abstract] [Full Text] [Related] [New Search]