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  • Title: A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing.
    Author: Umeda K, Seto Y, Kohda T, Mukamoto M, Kozaki S.
    Journal: Microbiol Immunol; 2010 May; 54(5):308-12. PubMed ID: 20536728.
    Abstract:
    A rapid, simple and sensitive multiplex PCR method for boNT/A gene cluster typing was developed by combining the results of BoNT/A subtype (boNT/A1 or /A2) gene detection with ha33 and/or p47 gene detection. Ten isolates associated with infant botulism in Japan were examined and divided into boNT/A gene cluster types 2 and 3 by origin (honey feeding or not) and period (1986-1987 or 1999-2007). It is suggested that this multiplex PCR method will be be useful for epidemiological studies of botulism.
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