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  • Title: [Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography].
    Author: Liu Z, Chou G, Wang Z.
    Journal: Zhongguo Zhong Yao Za Zhi; 2010 Mar; 35(6):729-31. PubMed ID: 20545198.
    Abstract:
    OBJECTIVE: To establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata. METHOD: The analysis was carried out on a Boston Crest ODS column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water as mobile phases. The flow rate was 1.0 mL x min(-1) and the detection wavelength was at 215 nm. RESULT: The calibration curve was linear over the range of 0.0202-20.02 microg for kirenol. The correlation coefficient of the calibration curve was 0.99975. The average recovery was 99.62% with relative standard derivation (RSD) of 2.0%. CONCLUSION: The results showed that the method is simple, accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.
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