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  • Title: Au NPs-enhanced surface plasmon resonance for sensitive detection of mercury(II) ions.
    Author: Wang L, Li T, Du Y, Chen C, Li B, Zhou M, Dong S.
    Journal: Biosens Bioelectron; 2010 Aug 15; 25(12):2622-6. PubMed ID: 20547052.
    Abstract:
    We reported a sensitive surface plasmon resonance (SPR) sensor for the detection of Hg(2+) in aqueous solution by using a thymine (T)-rich, mercury-specific oligonucleotide (MSO) probe and gold nanoparticles (Au NPs)-based signal amplification. The MSO probe was first immobilized on gold film through formation of Au-S bond between DNA and gold film. In the presence of Hg(2+), the MSO probe captured free Hg(2+) in aqueous media via the Hg(2+)-mediated coordination of T-Hg(2+)-T base pairs. This direct immobilization strategy led to a detection limit of 0.3 microM of Hg(2+). In order to improve the sensitivity, part complementary DNA (PCS)-modified Au NPs labels were employed to amplify SPR signals. We demonstrated that this Au NPs-based sensing strategy resulted in a detection limit down to 5 nM of Hg(2+), brings about an amplification factor of two orders of magnitude. This Au NPs-based Hg(2+) sensor also exhibited excellent selectivity over a spectrum of interference metal ions. Taking advantage of the high amplifying characteristic of Au NPs and the specificity of MSO to Hg(2+) recognition, we developed here a SPR sensor for specific Hg(2+) detection with high sensitivity.
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