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  • Title: A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection.
    Author: Zhou Y, Huang Q, Gao J, Lu J, Shen X, Fan C.
    Journal: Nucleic Acids Res; 2010 Aug; 38(15):e156. PubMed ID: 20547593.
    Abstract:
    We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sensitivity of D-RCA compares favorably with other existing technologies. More significantly, the dynamic range of D-RCA is extremely large, covering eight orders of magnitude. We also demonstrate miRNA quantification with this highly sensitive and inexpensive D-RCA strategy in clinical samples.
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