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  • Title: Using quantum dots as fluorescence probe to evaluate the immune complex deposits in paraffin-embedded sections.
    Author: Zhu J, Chen H, Ding G, Zhu X, Zhu R, Yang H.
    Journal: Clin Nephrol; 2010 Jul; 74(1):33-8. PubMed ID: 20557864.
    Abstract:
    OBJECTIVE: To detect the immune complex deposits in IgA nephropathy (IgAN) by using quantum dots (QDs)-labeled immunofluorescence technology and to evaluate its significance. METHODS: Using the fluorescence property of QDs and indirect immunofluorescence methods to detect the IgA and complement C3 in formalin-fixed paraffin-embedded (FFPE) tissues of IgAN. Minimal change disease (MCD) was used as a control. RESULTS: Immunoglobulin and a complement were significantly detected in IgAN and manifested as special distribution of intensive salmon pink fluorescence. Compared with fluorescein isothiocyanate (FITC) tagging, QD signal showed better specificity and signal to noise ratio, and QD signal was not significantly quenched after 60 min of excitation. CONCLUSION: QDs can be used to label subcellular proteins and have obvious advantages compared with the traditional organic fluorophores. QDs-tagged fluorescence can be applied as a new method for clinical labeling detection in renal pathology.
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