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  • Title: Comparison of serum versus plasma collection in gas chromatography--mass spectrometry-based metabolomics.
    Author: Dettmer K, Almstetter MF, Appel IJ, Nürnberger N, Schlamberger G, Gronwald W, Meyer HH, Oefner PJ.
    Journal: Electrophoresis; 2010 Jul; 31(14):2365-73. PubMed ID: 20564267.
    Abstract:
    Bovine serum, EDTA-plasma and EDTA-plasma fortified with acetylsalicylic acid (ASA) as antioxidant were compared with regard to their suitability for metabolomic studies. Metabolic fingerprints were generated from GC-TOF-MS data using the Leco ChromaTOF software in combination with the in-house retention time correction and data alignment tool INCA. A total of 6, 9 and 21 significant features with a false discovery rate of <0.05 were identified by INCA upon comparing EDTA- versus EDTA-ASA-plasma, EDTA-plasma versus serum and EDTA-ASA-plasma versus serum, respectively. To confirm that the observed signal intensities in the GC-TOF-MS fingerprints reflected true metabolite abundances, 19 amino acids, glucose and 6 organic acids were quantified by means of GC-MS using stable-isotope-labeled internal standards. As observed with the fingerprints, only the concentrations of lactate and citrate were found to be significantly lower in EDTA-plasma and serum, respectively, whereas the concentrations of the other metabolites were similar among the three sample types investigated.
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