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  • Title: Development of single-tube mutiplex RT-PCR for dengue virus typing.
    Author: Maneekan P, Jittmittraphap A, Leaungwutiwong P, Kaewkangwal J, Luplertlop N.
    Journal: Southeast Asian J Trop Med Public Health; 2009 Nov; 40(6):1254-8. PubMed ID: 20578459.
    Abstract:
    This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination.
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