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  • Title: Immobilization strategies for laccase from Trametes versicolor on mesostructured silica materials and the application to the degradation of naphthalene.
    Author: Fernando Bautista L, Morales G, Sanz R.
    Journal: Bioresour Technol; 2010 Nov; 101(22):8541-8. PubMed ID: 20599376.
    Abstract:
    The oxidation of naphthalene by immobilized laccase from Trametes versicolor has been performed using diverse immobilization strategies on mesostructured silica materials. Laccase was immobilized by physical adsorption on several SBA-15 with different textural properties and by covalent attachment on functionalized SBA-15 prepared by co-condensation method (direct synthesis). The adsorption of laccase was partially reversible and showed some degree of lixiviation. However, covalently attached laccase to aminopropyl and aminobutyl functionalized SBA-15 exhibited important activity for the degradation of naphthalene with, respectively, 35% and 39%wt of removal in 5h. The aminopropyl biocatalyst retained higher activity after repeated uses than the corresponding aminobutyl.
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