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  • Title: NAD nucleosidase of Agkistrodon bilineatus venom.
    Author: Brunson EM, Johnson BD, Sifford DH.
    Journal: Am J Trop Med Hyg; 1978 Mar; 27(2 Pt 1):365-8. PubMed ID: 206161.
    Abstract:
    NAD nucleosidase (NAD glycohydrolase, EC 3.2.2.5) activity in Agkistrodon bilineatus venom was observed. Using the cyanide assay method at pH 7.9, lyophilized crude venom had an activity of 0.19 units/mg. Chromatography of the crude venom on DEAE Sephadex A-50 with ammonium acetate buffer by two stage elution yielded 13 fractions. Peak NAD nucleosidase activity occurred at Fraction X. Crude venom and Fraction X NAD nucleosidase activities were thermolabile.
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