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  • Title: [Properties of urease immobilized by chemical binding to a derivatives of cellulose].
    Author: Fesenko RI, Artemova IuV, Malakhov AA, Virnik AD, Iakovlev VA.
    Journal: Prikl Biokhim Mikrobiol; 1977; 13(4):572-6. PubMed ID: 20617.
    Abstract:
    A comparative study of properties of soluble urease and urease immobilized by its binding to cellulose derivatives was carried out. Upon chemical binding of urease to cellulose derivatives the Mikhaelis constant and pH optimum for the enzyme action changed insignificantly. Maximum enzymic activity of soluble and immobilized urease occurred at 50 degrees C. The energy of activation of urea hydrolysis by urease during enzyme immobilization also changed insignificantly.
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