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  • Title: Comparative analysis of direct fluorescence, Zenon labeling, and quantum dot nanocrystal technology in immunofluorescence staining.
    Author: Tang X, He J, Partin J, Vafai A.
    Journal: J Immunoassay Immunochem; 2010; 31(3):250-7. PubMed ID: 20623410.
    Abstract:
    A comparative analysis was performed to determine the sensitivity and efficiency of three fluorescent labeling techniques, including direct fluorescent-antibody staining (FA), Zenon labeling, and quantum dot (QD) nanocrystal technology. Two varicella-zoster virus immunoglobin (Ig) G forms, mAb 4F9 and mAb g62, were selected for these studies. The results indicated that: (1) All three methods demonstrated similar brightness and photostability; (2) the time required to conjugate the antibody varied, with Zenon labeling being the quickest; and (3) the stability of each conjugated complex was different, with FITC/rhodamine-conjugated antibody being the most stable.
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