These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.
    Author: Monaco AP, Lam VM, Zehetner G, Lennon GG, Douglas C, Nizetic D, Goodfellow PN, Lehrach H.
    Journal: Nucleic Acids Res; 1991 Jun 25; 19(12):3315-8. PubMed ID: 2062647.
    Abstract:
    A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapped by this method, using a cosmid reference library for comparisons between overlapping hybrids to identify interesting clones for further analysis.
    [Abstract] [Full Text] [Related] [New Search]