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  • Title: Chlorpyrifos induces apoptosis and DNA damage in Drosophila through generation of reactive oxygen species.
    Author: Gupta SC, Mishra M, Sharma A, Deepak Balaji TG, Kumar R, Mishra RK, Chowdhuri DK.
    Journal: Ecotoxicol Environ Saf; 2010 Sep; 73(6):1415-23. PubMed ID: 20627310.
    Abstract:
    The present study investigated the apoptosis and DNA damage inducing potential of chlorpyrifos (CP) in Drosophila melanogaster. Third instar larvae of Drosophila were treated with different concentrations of CP (0.015-15.0 microg/L) for 2-48 h. Reactive oxygen species (ROS) generation, oxidative stress markers, DNA damage and apoptotic cell death end points were measured in them. A significant increase in DNA damage was concomitant with apoptotic mode of cell death in 15.0 microg/L CP-treated organisms for 24 and 48 h. Depolarization in mitochondrial membrane potential and increased casapase-3 and caspase-9 activities in these organisms indicated both as potential targets of CP. A significant positive correlation was observed among ROS generation, apoptosis and DNA damage. The study suggests that (i) ROS may be involved in inducing apoptosis and DNA damage in the CP-exposed larvae of Drosophila and (ii) D. melanogaster may be used as an alternative in vivo animal model for xenobiotics hazard assessment.
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