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  • Title: Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis.
    Author: Tong SY, Lilliebridge RA, Holt DC, Coombs GW, Currie BJ, Giffard PM.
    Journal: Diagn Microbiol Infect Dis; 2010 Aug; 67(4):399-401. PubMed ID: 20638613.
    Abstract:
    We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform.
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