These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Cloning of hsa-miR-148a and construction of its retroviral expression vector].
    Author: Xu XH, Quan TY, Zeng WX, Chen XJ, Li WM.
    Journal: Nan Fang Yi Ke Da Xue Xue Bao; 2010 Jul; 30(7):1545-7, 1557. PubMed ID: 20650762.
    Abstract:
    OBJECTIVE: To clone hsa-miR-148a and construct its retroviral expression vector. METHODS: The pre-miR-148a amplified by PCR was inserted to pMSCV to construct the recombinant retroviral expression plasmid pMSCV-miR-148a, which was confirmed by restriction endonuclease analysis and DNA sequencing. The retroviral expression vector pMSCV-miR-148a and PIK packaging plasmid were cotransfected into 293FT packaging cells by calcium phosphate-mediated transfection to produce the retrovirus, and the retrovirus titer was measured by infection of NIH3T3 cells. RESULTS: Restriction enzyme digestion and DNA sequencing demonstrated that the retroviral vector pMSCV-miR-148a was constructed successfully, and the virus titer was 5x10(8) CFU/ml after infection of NIH3T3 cells. CONCLUSION: The successful construction of the retroviral expression vector MSCV-miR-148a allows the production of high-titer retrovirus to facilitate further study of the molecular functions of miR-148a.
    [Abstract] [Full Text] [Related] [New Search]