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Title: The frequencies of transcription from the E- and L-strands of polyoma DNA. Author: Bar-Shavit R, Laub O, Aloni Y. Journal: J Gen Virol; 1978 May; 39(2):357-60. PubMed ID: 206660. Abstract: Exhaustion type hybridization was used to measure the amount of nuclear virus RNA complementary to the early (E) and late (L) polyoma virus DNA strands. At 36 h after infection between 2.5 and 7.3% of the newly synthesized virus RNA was complementary to the E-strand (-strand) and between 92.7 and 97.5% was complementary to the L-strand (+strand). This proportion was independent of the labelling time, indicating similar accumulation of the E- and L-RNA transcripts in the nucleus. The nuclear E- and L-RNA transcripts sedimented in a similar manner through sucrose gradients.[Abstract] [Full Text] [Related] [New Search]