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  • Title: Accumulation of selenium and changes in the activity of inulinase and catalase in the cells of Kluyveromyces marxianus on pulsed electric field treatment.
    Author: Pankiewicz U, Jamroz J.
    Journal: J Microbiol Biotechnol; 2010 Jul; 20(7):1101-6. PubMed ID: 20668403.
    Abstract:
    Pulsed electric field (PEF) of 1Hz, 1.5 kV, and 1 ms increased the activities of catalase and inulinase over the whole range of applied Se concentrations compared with the non-treated cultures. A significant effect of selenium concentration (in the range of 5-14 microg/ml) on both intra- and extracellular enzyme activities was noted. At a Se concentration of 10 microg/ml, the activities of intra- and extracellular inulinases and extracellular catalase in the PEF-treated cultures reached the maximum of 71 U/g d.m., 46 U/g d.m., and approx. 8 U/ml, respectively. The maximum activity of intracellular catalase of approx. 6 U/ ml (with and without PEF) was recorded at 5 microg Se/ml. Further increasing of selenium concentration caused a decrease in the activity of the enzymes.
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