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  • Title: [Plasmid R68.45 and S-a transduction by the temperate bacteriophage 59 of Erwinia carotovora 268].
    Author: Mukvich NS, Romaniuk LV, Maliuk AG, Shevchenko TV, Kishko IaG.
    Journal: Mikrobiol Zh (1978); 1991; 53(1):28-33. PubMed ID: 2067419.
    Abstract:
    Temperature bacteriophage 59 of Erwinia carotovera 268 had transduced extrachromosomal DNA: plasmids of R68.45 and S-a. Before plasmid transduction experiments the suitable donor strains of indicator culture Erwinia horticola 450 harbouring R68.45 and S-a were created. The frequency of plasmid R68.45 transfer from Pseudomonas putida to E. horticola 450-8 by conjugation was equal to 5 x 10(-8) per a donor cell and in the case of S-a--from E. coli C600 for the same recipient cells--was 2 x 10(-6). Bacteriophage 59 has transduced only separate markers of plasmid R68.45, since plasmid S-a is probably transduced by the phage as an intact unit.
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