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  • Title: [Studies on NACE-DAD fingerprint of Cortex Phellodendri].
    Author: Li JS, Liu XH, Cai BC, Fu XS.
    Journal: Zhong Yao Cai; 2010 Mar; 33(3):349-52. PubMed ID: 20681296.
    Abstract:
    OBJECTIVE: To establish the analytical method for the fingerprint of Cortex Phellodendri by NACE-DAD and estimate the quality of Cortex Phellodendri from different habitats and species. METHODS: Based on the mode of nonaqueous capillary electrophoresis, 40 mmol/L sodium acetate and 40 mmol/L ammonium acetate methanol solution was selected for the buffer (pH 5.8). The separation voltage was 25 kV and detection wavelength was set at 210 nm. Berberine was used as a reference standard, the chromatographic fingerprint was determined. The data were analysed by Fuzzy Cluster and Fingerprint Similarity Evaluation Software to compare the similarity of samples. RESULTS: NACE-DAD fingerprints with 9 common peaks were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Cortex Phellodendri and its processed products, there were obvious differences in the relative areas of common peaks. CONCLUSION: The method is reliable, accurate and can be used for quality control of Cortex Phellodendri.
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