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  • Title: High-resolution local imaging of temperature in dielectrophoretic platforms.
    Author: Gielen F, Pereira F, Demello AJ, Edel JB.
    Journal: Anal Chem; 2010 Sep 01; 82(17):7509-14. PubMed ID: 20684541.
    Abstract:
    The use of dielectrophoretic forces is crucially tied to the knowledge of Joule heating within a fluid, since the use of planar microelectrodes creates a temperature gradient within which the particle of interest is manipulated. Mapping temperature with sufficient spatial resolution within a dielectrophoretic trap is recognized to be of high importance. Herein, we demonstrate local temperature measurements in the vicinity of a trapped micrometer-size particle using confocal fluorescence spectroscopy. Such measurements are shown to provide a novel calibration tool for screening temperature-mediated processes with high resolution.
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