These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2.
    Author: Cheng X, Lu G, Qi J, Cheng H, Gao F, Wang J, Yan J.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2010 Aug 01; 66(Pt 8):909-12. PubMed ID: 20693665.
    Abstract:
    Phosphoribosylaminoimidazole-succinocarboxamide synthase (SAICAR synthase) plays an essential role in the de novo biosynthesis of purine nucleotides. In this study, the SAICAR synthase from Streptococcus suis was cloned and overexpressed in Escherichia coli. The subsequent product was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.8 A resolution and belonged to space group P2, with unit-cell parameters a=70.2, b=52.2, c=153.9 A, beta=102.8 degrees.
    [Abstract] [Full Text] [Related] [New Search]