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  • Title: Quantitative studies of sulphate conjugation by isolated rat liver cells using [35S]sulphate.
    Author: Dawson J, Knowles RG, Pogson CI.
    Journal: Biochem Pharmacol; 1991 Jun 21; 42(1):45-9. PubMed ID: 2069597.
    Abstract:
    We have developed a simple, rapid and sensitive method for the study of sulphate conjugation in isolated liver cells based on the incorporation of 35S from [35S]sulphate. Excess [35S]sulphate is removed by a barium precipitation procedure, leaving [35S]sulphate conjugates in solution. We have used this method to examine the kinetics of sulphation of N-acetyl-p-aminophenol (acetaminophen), 4-nitrophenol and 1-naphthol in isolated rat liver cells. The efficiency of recovery of the sulphate conjugates was greater than 86%. The method is applicable to the quantitative study of sulphate conjugation of any substrate which forms a sulphate conjugate that is soluble in the presence of barium, without the need for standards or radiolabelled sulphate acceptors.
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