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  • Title: Prolonged expression of biotransformation activities of rat hepatocytes co-cultured with established cell lines.
    Author: Donato MT, Castell JV, Gómez-Lechón MJ.
    Journal: Toxicol In Vitro; 1990; 4(4-5):461-6. PubMed ID: 20702215.
    Abstract:
    Co-culture of hepatocytes with fibroblastic cells (C3H/10T1 2 and 3T3) allowed the expression of phase I and II biotransformation enzymes for a prolonged time in culture. After 7 days in co-culture, cytochrome P-450 and monooxygenase activities were still 20-30% of the initial value, while in pure hepatocyte cultures they were undetectable. NADPH-cytochrome c reductase and conjugation activities remained at nearly initial levels for at least 7 days in co-cultured hepatocytes. Finally, in co-cultures a clear and prolonged induction of monooxygenase activities by 3-methylcholanthrene and phenobarbital was observed.
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