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  • Title: [Spectra analysis of lignin small molecular guaiacyl coniferyl alcohol biological modification treated by laccase].
    Author: Liu HT, Pei JC, Hu HR, Pei Y.
    Journal: Guang Pu Xue Yu Guang Pu Fen Xi; 2010 Jun; 30(6):1469-73. PubMed ID: 20707131.
    Abstract:
    The enzymatic modification mechanism of lignin small molecular lignin guaiacyl coniferyl alcohol existing in softwood and hardwood treated by laccase was studied. Gas chromatography-mass spectrometry (GC-MS), gel permeation chromatography (GPC), FTIR spectrum and PCD(particle charge detector) etc were used for the measurement. GC-MS could not detect the coniferyl alcohol monomer after treated by laccase, so it was speculated that coniferyl alcohol participated in the reaction totally, and the structure of coniferyl alcohol was changed. GPC demonstrated that the molecular weight of coniferyl alcohol increased when it was treated by laccase, and coniferyl alcohol was polymerized. FTIR spectrum determined the reaction point of coniferyl alcohol when treated by laccase, and it is mainly phenolic hydroxyl, aromatic ring side chain substituent such as methoxy, double bound in side chain, beta-carbonyl groups, and gamma-carbonyl groups. PCD gave a result that the cationic demandv(CD) decreased by 88.38%.
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